Composite

Part:BBa_K2557022:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


miniCMV promoter-PhiC31-RDF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 46
    Illegal BamHI site found at 957
    Illegal BamHI site found at 1043
    Illegal BamHI site found at 1290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 124
    Illegal NgoMIV site found at 353
    Illegal NgoMIV site found at 395
    Illegal NgoMIV site found at 1966
    Illegal AgeI site found at 52
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 649
    Illegal BsaI.rc site found at 1052
    Illegal BsaI.rc site found at 1405
    Illegal BsaI.rc site found at 2047
    Illegal SapI.rc site found at 2649


Design Notes

In order to allow the recombinase to enter the nucleus and function, we added SV40 NLS to the front end. To detect protein content, we added the FLAG tag.


Source

The miniCMV promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the sequence of PhiC31-RDF from the literature and synthesized this sequence.

References